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5x iscript reverse transcriptase  (Qiagen)


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  • 97

    Structured Review

    Qiagen 5x iscript reverse transcriptase
    5x Iscript Reverse Transcriptase, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+iscript+reverse+transcriptase/Reverse+transcriptase/pmc06043659-104-42-19
    Average 97 stars, based on 6786 article reviews
    5x iscript reverse transcriptase - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Generated:

    Article Title: Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach
    Article Snippet: Secondary Antibody- Peroxidase Affinipure Donkey Anti-Rabbit (Cat #711-035-152, Jackson Immunoresearch Labs) .. For each stage of stem cell development, colonies were picked and mRNA was extracted using the miRNeasy Mini Kit (QIAGEN® cat. No. 217004). cDNA was generated via the iScript cDNA synthesis kit (BioRad Cat #1708890) using a 20μl mixture (5x Supermix (4μl), 5x iScript reverse transcriptase (1μl), Nuclease-free water (5μl), and RNA template (10μl). .. RT-PCR analysis was performed by using a 22μl mixture (MyTaq Supermix (BIOLINE PCR Kit®) 12μl, Forward (2μl) and Reverse (2μl) primers, (2μl) Nuclease-free water, (4ul) cDNA template).

    cDNA Synthesis:

    Article Title: Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach
    Article Snippet: Secondary Antibody- Peroxidase Affinipure Donkey Anti-Rabbit (Cat #711-035-152, Jackson Immunoresearch Labs) .. For each stage of stem cell development, colonies were picked and mRNA was extracted using the miRNeasy Mini Kit (QIAGEN® cat. No. 217004). cDNA was generated via the iScript cDNA synthesis kit (BioRad Cat #1708890) using a 20μl mixture (5x Supermix (4μl), 5x iScript reverse transcriptase (1μl), Nuclease-free water (5μl), and RNA template (10μl). .. RT-PCR analysis was performed by using a 22μl mixture (MyTaq Supermix (BIOLINE PCR Kit®) 12μl, Forward (2μl) and Reverse (2μl) primers, (2μl) Nuclease-free water, (4ul) cDNA template).

    Reverse Transcription:

    Article Title: Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach
    Article Snippet: Secondary Antibody- Peroxidase Affinipure Donkey Anti-Rabbit (Cat #711-035-152, Jackson Immunoresearch Labs) .. For each stage of stem cell development, colonies were picked and mRNA was extracted using the miRNeasy Mini Kit (QIAGEN® cat. No. 217004). cDNA was generated via the iScript cDNA synthesis kit (BioRad Cat #1708890) using a 20μl mixture (5x Supermix (4μl), 5x iScript reverse transcriptase (1μl), Nuclease-free water (5μl), and RNA template (10μl). .. RT-PCR analysis was performed by using a 22μl mixture (MyTaq Supermix (BIOLINE PCR Kit®) 12μl, Forward (2μl) and Reverse (2μl) primers, (2μl) Nuclease-free water, (4ul) cDNA template).



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